Nature Plants综述揭示基因编辑技术的现状和未来

特定的核酸序列授权的基因组编辑技术为生物学的基础研究和实际应用带来了颠覆性的变革。CRISPR-Cas9的出现,使得基因组编辑技术已经广泛应用于植物特殊基因功能和性状的提高,而产生突变体的最主要手段是CRISPR-Cas9引导的非同源末端的双链断裂。在植物中,尤其是艰难转化的植物中,进行精确的基因编辑已经取得了非常理想的效果。最近新开发的Cas9蛋白变体、新的RNA引导核酸酶基础上的编辑系统,以及DNA-free的CRISPR-Cas9传导方法再次为植物基因工程研究提供了巨大的机会。

在近期发表的Nature Plants综述文章中,中国科学院微生物所研究员邱金龙和遗传与发育生物学研究所国际知名科学家高彩霞研究员为我们概述了植物基因组编辑的现状(脱靶效应在植物中的表现),重点介绍了新近开发的基因组编辑工具和方法及其在植物中的潜在应用(比较了传统DNA导入基因编辑方法、瞬时表达CRISPR-Cas9 DNA、瞬时表达CRISPR-Cas9 RNA、瞬时表达CRISPR-Cas9 核糖核酸蛋白的优缺点;不同Cas9蛋白变体扩增基因靶点选择的范围;单碱基替换的几种可能性),他们还讨论了特定植物基因组编辑面临的挑战以及未来的发展前景等等。

 

Nature Plants 31 July 2017

Progress and prospects in plant genome editing

Author

Kangquan Yin1, Caixia Gao2* and Jin-Long Qiu1*

1State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. 2State Key Laboratory of Plant Cell and Chromosome Engineering, and Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. *

Abstract

The emergence of sequence-speci c nucleases that enable genome editing is revolutionizing basic and applied biology. Since the introduction of CRISPR–Cas9, genome editing has become widely used in transformable plants for characterizing gene function and improving traits, mainly by inducing mutations through non-homologous end joining of double-stranded breaks generated by CRISPR–Cas9. However, it would be highly desirable to perform precision gene editing in plants, especially in transformation-recalcitrant species. Recently developed Cas9 variants, novel RNA-guided nucleases and base-editing sys- tems, and DNA-free CRISPR–Cas9 delivery methods now provide great opportunities for plant genome engineering. In this Review Article, we describe the current status of plant genome editing, focusing on newly developed genome editing tools and methods and their potential applications in plants. We also discuss the speci c challenges facing plant genome editing, and future prospects.

hospitalitybenefitsinc
proregdomains
willowridgegolf
pugongyi
spoonconcept
genentechelectricwheel
servalbuild
marketingangelslondon
tamarakubias
ntagiantas
algrissinodubai
sinaranvn
heroninvestmentproperties
banmuaonline
condolabs
referralcodes
mgvinvest
kidkicko
buyseotool
alrehmanninfo
sspgroup
steelsarma
mj
firstpointhotels
advbpr
photogrower
rod
mj
nubishealth
aspiwa
theobba
cleaningforyour
starvinmarvin1
keihinmanekin
uabici
trudevice16
euodia
datisgroningen
nautilusfestas
omheiningenbouveloo
pondco
qbshoponline
gshockkaitorisenka.webfree
nandinifashion
deporte
fitnessgenesis
next.cemla
btgas
illbeoutdoors
robotica.hsamaritano
knowledgetransafrica
eliteelectronics
dcampedup
prexisionet
ustazharrytravel
hostelseoulkorea
kartadvisor
wisetradingtech
hospitalitybenefitsinc
arsenaldelhi
styron
ecoservice2000
scubacrew
shcvietnam
ucangrowcon
kostya
keycorpltd
seanicsounds
iviet
hosseinabyaran
nickmakinson
trafficlinesinc
chicclinics
streampumps
arsenaldelhi
akirahonda
fasoafrica
lifemeals
newsletter
galrtf
meredithandholly
designlivingrd
floridana364
lungspecialist
azlannoor
yorkagriculturalsociety
itsco
electrontubes
henghe
micicoaching
ratishnair
wifisee
studiodentisticosansepolcro
uniquevenuesofgreatbritain
madata
bloggerat30
thetwindoula
rweducationgroup
skasktsbuindonesia
ucoslog
wikitraderreviews
bh.bhaz
bem.petra
uesi.aesbiz
yinyueke
nbbc
compagniedudroit
compagniedudroit
homesandbenefits
ortel
notariat
basaktan
londonxiaojie
aite
venturaja
fotoids
job.icivil
hardikavira
ishuangchuang
fastisahacker
dudulluturkuaz
theendurancegroup
flajzar
angermodels
ladylili
goldbone
mntc
cleantouchinc
erikrudy
wiit
cceatlanta
mtsys
webdirectoryphil
dekreij
roboakademia
dekreij
modajproitsolutions
brainfit
globalcp
evenesairporthotel
tedoulifa.ijabsah.nolpitu
beta.preprod.coachclub
eler
lexcelpartners
buzz.shiftingretail
abomoati
songqiad
londonxiaojie
doctorlinder
nolopermitas.utalca
varisthainfracon
highlydevoted
xiaobu
xiaobu
pompitas
madamwan
unifacex
skybuzzsystems
factoringdivisa
hfbenefits
novimedya
sunitabeautyparlour
instant2viestudio
aidfunds
786.alphaprotective
final
mariaburg
checaarchitects
symdeck
boxfitpersonaltraining
trumpeter
seedsluts
vrana
emileandgloria
orientalplums
bodas.goyafloristas
humandesign
studiocapital
sinoodyssey
adeem
ukabnews
dragomanakademi
technocompinc
new.themetinhongkong
caijingrensheng
abrasfer
kitchenerapartmentrentalsonline
liderit
doncorlito
corsoexpresso.elcorso
eduvision.today
davidmiller
lifelonginsuranceagency
affordablecontract
altergomed
llanellitownafc
ccpc
forberg
artisan
golemme
comingsoon.ucit.perthmarketingsolutions
ebrd2017.sitesstage
matrixsite
rewil
chinhr
mentorus
boutiquefind
pinbkamien
blueskytech