美BHB公司开发新的CRISPR核酸酶,用于高效的基因精准插入和替换

为了扩大基因编辑技术的应用领域,实现高效的基因精准插入和碱基替换,来自美国BHB公司的研发团队测试了弗朗西斯氏菌和毛螺菌中的Cpf1核酸酶。结果显示,这些Cpf1核酸酶在水稻基因组中的精准敲入效率可以达到8%。

Benson Hill Biosystems(BHB)是一家提供生物信息云平台服务的创新企业,其建立的CropOS™生物信息平台收集了大量的基因数据,并通过生物智能技术去精准的寻找那些可以改良农作物性状的基因,这些性状包括增产、抗逆、抗病、营养品质等各方面。截至目前,BHB的服务对象包括美国农业部、美国大豆协会、华盛顿州立大学、博伊斯汤姆森研究所、北卡生物技术中心、Iowa Corn委员会等研究机构以及利马格兰Limagrain、辛普劳simplot、美棉Cotton Incorporated等知名公司。随着基因编辑技术的兴起,BHB也开启了新的业务板块。BHB利用Cms1核酸酶开发的CRISPR 3.0基因编辑技术,相对于普遍使用的CRISPR/Cas9而言,具有效率更高、知识产权更清晰的优势。

2017年初,BHB公司被美国农业投资媒体Agfounder评为美国最值得关注的十大农业创业公司之一。

 

Scientific Reports, 7: 11606; 14 September 2017

Precise insertion and guided editing of higher plant genomes using Cpf1 CRISPR nucleases

Author

Matthew B. Begemann, Benjamin N. Gray, Emma January……Thomas P. Brutnell, Todd C. Mockler & Mohammed Oufattole

Benson Hill Biosystems, USA

Abstract

Precise genome editing of plants has the potential to reshape global agriculture through the targeted engineering of endogenous pathways or the introduction of new traits. To develop a CRISPR nuclease-based platform that would enable higher efficiencies of precise gene insertion or replacement, we screened the Cpf1 nucleases from Francisella novicida and Lachnospiraceae bacterium ND2006 for their capability to induce targeted gene insertion via homology directed repair. Both nucleases, in the presence of a guide RNA and repairing DNA template flanked by homology DNA fragments to the target site, were demonstrated to generate precise gene insertions as well as indel mutations at the target site in the rice genome. The frequency of targeted insertion for these Cpf1 nucleases, up to 8%, is higher than most other genome editing nucleases, indicative of its effective enzymatic chemistry. Further refinements and broad adoption of the Cpf1 genome editing technology have the potential to make a dramatic impact on plant biotechnology.