近日，Scientific Reports在线发表日本科学家Ryozo Imai团队题为“An in planta biolistic method for stable wheat transformation”研究论文，该研究以小麦成熟胚分生组织为受体材料，利用基因枪方法获得稳定转基因植株，该种方法并不需要愈伤诱导，再生等繁琐步骤。
An in planta biolistic method for stable wheat transformation
Haruyasu Hamada1,2, Qianyan Linghu1, Yozo Nagira2, Ryuji Miki2, Naoaki Taoka2 & Ryozo Imai1,3
The currently favoured method for wheat (Triticum aestivum L.) transformation is inapplicable to many elite cultivars because it requires callus culture and regeneration. Here, we developed a simple, reproducible, in planta wheat transformation method using biolistic DNA delivery without callus culture or regeneration. Shoot apical meristems (SAMs) grown from dry imbibed seeds were exposed under a microscope and subjected to bombardment with different-sized gold particles coated with the GFP gene construct, introducing DNA into the L2 cell layer. Bombarded embryos were grown to mature, stably transformed T0 plants and integration of the GFP gene into the genome was determined at the fifth leaf. Use of 0.6-μm particles and 1350-psi pressure resulted in dramatically increased maximum ratios of transient GFP expression in SAMs and transgene integration in the fifth leaf. The transgene was integrated into the germ cells of 62% of transformants, and was therefore inherited in the next generation. We successfully transformed the model wheat cultivar ‘Fielder’, as well as the recalcitrant Japanese elite cultivar ‘Haruyokoi’. Our method could potentially be used to generate stable transgenic lines for a wide range of commercial wheat cultivars.